Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis
نویسندگان
چکیده
منابع مشابه
Development of a multiplex PCR assay for detection and genogrouping of Neisseria meningitidis.
Neisseria meningitidis is a leading pathogen of epidemic bacterial meningitis and fulminant sepsis worldwide. Twelve different N. meningitidis serogroups have been identified to date based on antigenic differences in the capsular polysaccharide. However, more than 90% of human cases of N. meningitidis meningitis are the result of infection with just five serogroups, A, B, C, W135, and Y. Effici...
متن کاملsodC-Based Real-Time PCR for Detection of Neisseria meningitidis
Real-time PCR (rt-PCR) is a widely used molecular method for detection of Neisseria meningitidis (Nm). Several rt-PCR assays for Nm target the capsule transport gene, ctrA. However, over 16% of meningococcal carriage isolates lack ctrA, rendering this target gene ineffective at identification of this sub-population of meningococcal isolates. The Cu-Zn superoxide dismutase gene, sodC, is found i...
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15 صفحه اولComparison of susceptibility of Neisseria meningitidis
When prevalence of meningococcal disease is increased contacts of cases may require chemoprophylaxis, and sodium sulphadiazine is the drug of choice. However, increasing numbers of meningococci are resistant to sulphonamides (Fallon, 1975, 1977) andmany alternative chemoprophylactic agents are ineffectual. Sodium fusidate is active against a few strains of meningococci in vitro (Barber and Wate...
متن کاملNeisseria meningitidis storage identification and MIC determination.
The significance Minimum Inhibitory Concentration (MIC) of determination for Meningococci is described and according to the present study a shift in the sensitivity of Meningococcus is occurring, local isolates show higher Minimum Inhibitory Concentration for Chloramphenicol and Azactam. Strains of Meningococcus can successfully be stored in a domestic freezer.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2005
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.43.1.144-149.2005